Mass Spectrometry Facility provides services for identification and quantification of small and large molecules like secondary metabolites, peptides and proteins. The facility houses ESI-MS: TripleTOF® 5600+; MALDI TOF/ TOF Mass Spectrometer: SCIEX TOF/TOFTM 5800; ESI-Mass Spectrometer: SCIEX QTRAP® LCMS/MS 6500+ and Perkin Elmer FlexarTM HPLC system.
1. ESI-Mass Spectrometer: SCIEX TripleTOF® 5600+
Mass Spectrometry enables determination of the molecular masses of small molecules as well as of large biomolecules such as proteins and peptides. The SCIEX Triple TOF 5600+ mass spectrometer is a high-resolution LC-MS/MS mass spectrometer with high sensitivity and high mass accuracy. The Triple TOF® 5600+ has both ESI and APPI ionization sources and is hyphenated with an ExionLC for LC-MS analysis of small molecules and also with an Eksigent ekspertnanoLC 425 for qualitative and quantitative proteomics analysis.
2. MALDI TOF/ TOF Mass Spectrometer: SCIEX TOF/TOFTM 5800
The facility also houses SCIEX TOF/TOFTM 5800 mass spectrometer with EKSpot MALDI spotter for qualitative and quantitative proteomics analysis. The system is widely used for identification of protein/peptide, characterization of PTM, de novo sequencing and relative quantification using iTRAQ, SILAC, TMT etc.
3. ESI-Mass Spectrometer: SCIEX QTRAP® LCMS/MS 6500+
The facility is equipped with SCIEX QTRAP® LCMS/MS 6500+ system. It is the fastest and most sensitive QTRAP. It provides the best LOD's and LOQs to enable the detection and quantification of the widest scope of chemical compounds in the most challenging matrices. Improved polarity switching and MRM3 speeds allow faster chromatography and better throughput. The built in QTRAP functionality enables quantitative MRMs and qualitative QTRAP scans in the same injection to maximize throughput. Best being utilized for performing applications requiring ultimate sensitivity for ultra-low-level identification and quantitation of both small and large molecules and for confirmatory analysis of metabolites.
4. Perkin Elmer FlexarTM HPLC system
The facility provides services for cation exchange/high pH fractionation of complex proteomics samples using Perkin Elmer FlexarTM HPLC system which is coupled with automated fraction collector for wide coverage of proteome.
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